Type 2 diabetes (T2D) is characterized by decreased insulin sensitivity and beta cell dysfunction leading to impaired regulation of blood glucose. At the cellular level, the lack of normal insulin signaling leads to a dysregulation of glucose and fatty acid metabolism.
The RESET study performed at SDCC in the Pathophysiology & Prevention team in the Clinical Epidemiology group (link to Epi group/reset study on homepage here) investigates the effects of a 13-week time-restricted eating intervention in overweight individuals with a high risk of developing T2D. The hypothesis is that the study participants will lose weight and improve their blood glucose regulation and blood lipid profile in the course of the intervention.
As part of the RESET study, cellular experiments will be performed to examine how the metabolic activity is affected by the intervention as it is expected that the metabolic status of the study participants will be reflected at the cellular level. Blood cells are an easily accessible cell population to test this hypothesis, therefore we will analyse the peripheral blood mononuclear cells` (PBMC) capacity and efficiency of oxidative phosphorylation as well as their glycolytic activity, both before and after the intervention. We will also test the contribution of fatty acid metabolism to basal and maximal substrate oxidation. The results of these measurements should help understand the beneficial effects of the intervention.
The aim is to examine whether a 13-week time-restricted eating period causes changes to the PBMC metabolic activity thereby providing possible mechanistic insight into how systemic metabolism in overweight individuals is affected by the intervention.
Kristine Færch and Jonas Salling Quist, Clinical Epidemiology, SDCC